Journal: Journal of Lipid Research
Article Title: Naturally occurring monoepoxides of eicosapentaenoic acid and docosahexaenoic acid are bioactive antihyperalgesic lipids [S]
doi: 10.1194/jlr.M006007
Figure Lengend Snippet: Determination of the kinetic constants for 14,15-EpETE (A) and 13,14-EpDPE (B) with the human sEH([E]final ≈ 3 nM) in Bis-Tris HCl buffer (25 mM, pH 7.0) containing 0.1 mg/ml of lipid free BSA at 30°C. The kinetic constants (KM and Vm) were calculated by nonlinear fitting of the Michaelis equation using the enzyme kinetic module of SigmaPlot version 9.01 (Systat Software Inc., Chicago, IL).
Article Snippet: Kinetic parameters were determined by nonlinear regression of the Michaelis-Menten equation using SigmaPlot (v9.01, Systat Software Inc., Chicago, IL).
Techniques: Software